Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA; Ayako SUGII; Kazuaki SATO; Xiao-Yong ZHANG; An-Lan DAI; Goro TAGUCHI; Makoto SHIMOSAKA

doi:10.1271/bbb.110605

Biochemistry & Molecular Biology Notes

Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA, Ayako SUGII, Kazuaki SATO, Xiao-Yong ZHANG, An-Lan DAI, Goro TAGUCHI, Makoto SHIMOSAKA

著者情報

キーワード: Aspergillus oryzae, chitosanase, family 75 glycosyl hydrolase

ジャーナルフリー

2012 年 76 巻 1 号 p. 193-195

DOI https://doi.org/10.1271/bbb.110605

View "Advance Publication" version (January 7, 2012).

詳細

抄録

A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.

責任著者(Corresponding author)

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