Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA; Ayako SUGII; Kazuaki SATO; Xiao-Yong ZHANG; An-Lan DAI; Goro TAGUCHI; Makoto SHIMOSAKA

doi:10.1271/bbb.110605

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae

Akihiro SUGITA, Ayako SUGII, Kazuaki SATO, Xiao-Yong ZHANG, An-Lan DAI, Goro TAGUCHI, Makoto SHIMOSAKA

著者情報

キーワード: Aspergillus oryzae, chitosanase, family 75 glycosyl hydrolase

ジャーナルフリー早期公開

論文ID: 110605

DOI https://doi.org/10.1271/bbb.110605

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 1 p.193

詳細

抄録

A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）