A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA

Masato ISHIHARA; Etsuko NISHIMOTO; Shoji YAMASHITA; Yoshimitsu KAKUTA; Makoto KIMURA

doi:10.1271/bbb.120546

Biochemistry & Molecular Biology Notes

A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA

Masato ISHIHARA, Etsuko NISHIMOTO, Shoji YAMASHITA, Yoshimitsu KAKUTA, Makoto KIMURA

著者情報

キーワード: fluorescence resonance energy transfer, Pyrococcus horikoshii, RNA-binding protein, RNA chaperon, ribonuclease P proteins

ジャーナルフリー

2012 年 76 巻 12 号 p. 2335-2337

DOI https://doi.org/10.1271/bbb.120546

View "Advance Publication" version (December 7, 2012).

詳細

抄録

The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active conformation via a distinct mode of binding.

責任著者(Corresponding author)

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