Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon Pyrococcus horikoshii by Tobacco Chloroplast Engineering

Abstract

One of the most cost-effective methods of producing industrial enzymes is by the use of transgenic plants. We demonstrated successful high-level expression of a hyperthermostable archaeal β-1,4-endoglucanase in mature tobacco leaves by transformation of chloroplasts by homologous recombination. The active recombinant enzyme was readily recovered not only from fresh but also from dried leaves.