A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426

Hirokazu SUZUKI; Keisuke WADA; Megumi FURUKAWA; Katsumi DOI; Toshihisa OHSHIMA

doi:10.1271/bbb.130492

Microbiology & Fermentation Technology Notes

A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426

Hirokazu SUZUKI, Keisuke WADA, Megumi FURUKAWA, Katsumi DOI, Toshihisa OHSHIMA

著者情報

キーワード: Geobacillus kaustophilus, orotidine-5'-phosphate decarboxylase, pyrF, ternary conjugation, functional screening

ジャーナルフリー
電子付録

2013 年 77 巻 11 号 p. 2316-2318

DOI https://doi.org/10.1271/bbb.130492

View "Advance Publication" version (November 7, 2013).

詳細

抄録

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

責任著者(Corresponding author)

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