A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426

Hirokazu SUZUKI; Keisuke WADA; Megumi FURUKAWA; Katsumi DOI; Toshihisa OHSHIMA

doi:10.1271/bbb.130492

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A Ternary Conjugation System for the Construction of DNA Libraries for Geobacillus kaustophilus HTA426

Hirokazu SUZUKI, Keisuke WADA, Megumi FURUKAWA, Katsumi DOI, Toshihisa OHSHIMA

著者情報

キーワード: Geobacillus kaustophilus, orotidine-5'-phosphate decarboxylase, pyrF, ternary conjugation, functional screening

ジャーナルフリー早期公開
電子付録

論文ID: 130492

DOI https://doi.org/10.1271/bbb.130492

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013), No. 11 pp. 2316-2318

詳細

抄録

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

責任著者(Corresponding author)

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