Characterization of Polyphosphate Glucokinase SCO5059 from Streptomyces coelicolor A3(2)

Mai KOIDE; Akimasa MIYANAGA; Fumitaka KUDO; Tadashi EGUCHI

doi:10.1271/bbb.130498

Biochemistry & Molecular Biology Notes

Characterization of Polyphosphate Glucokinase SCO5059 from Streptomyces coelicolor A3(2)

Mai KOIDE, Akimasa MIYANAGA, Fumitaka KUDO, Tadashi EGUCHI

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Keywords: polyphosphate glucokinase, SCO5059, Streptomyces coelicolor A3(2), glucose 6-phosphate

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Supplementary material

2013 Volume 77 Issue 11 Pages 2322-2324

DOI https://doi.org/10.1271/bbb.130498

View "Advance Publication" version (November 7, 2013).

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Abstract

SCO5059, encoded in Streptomyces coelicolor A3(2), was identified as a polyphosphate glucokinase. The K_m values of SCO5059 for glucose and polyphosphate (poly(P)₆) were estimated to be 12 and 4 µM, respectively, and the k_cat value was 0.3 s⁻¹ at pH 7.7 at 28 °C. SCO5059 homologs are highly conserved among Streptomyces, and can work as polyphosphate glucokinase as well.

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