Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts

Yuki HAMAMOTO; Takuji TSUKIYAMA; Yoshihiro YOSHIKAKE; Masayoshi TERAISHI; Yutaka OKUMOTO; Takatoshi TANISAKA

doi:10.1271/bbb.130480

Biochemistry & Molecular Biology Notes

Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts

Yuki HAMAMOTO, Takuji TSUKIYAMA, Yoshihiro YOSHIKAKE, Masayoshi TERAISHI, Yutaka OKUMOTO, Takatoshi TANISAKA

著者情報

キーワード: rice, protoplast, transient reporter assay, translation efficiency, GVG system

ジャーナルフリー

2013 年 77 巻 12 号 p. 2480-2482

DOI https://doi.org/10.1271/bbb.130480

View "Advance Publication" version (December 7, 2013).

詳細

抄録

We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.

責任著者(Corresponding author)

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