Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Note
Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
Mado MIYAHARAMasaki AOINatsuko INOUE-KASHINOYasuhiro KASHINOKentaro IFUKU
Author information
JOURNALS FREE ACCESS

2013 Volume 77 Issue 4 Pages 874-876

Details
Abstract

A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 108 cells) is achieved.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top