Preparation of Protein Transduction Domain-Fused Peptidyl Prolyl cis/trans Isomerase Pin1

Tsubasa OHASHI; Sumito TESHIMA; Masafumi HIDAKA; Takafumi UCHIDA

doi:10.1271/bbb.100372

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Preparation of Protein Transduction Domain-Fused Peptidyl Prolyl cis/trans Isomerase Pin1

Tsubasa OHASHI, Sumito TESHIMA, Masafumi HIDAKA, Takafumi UCHIDA

著者情報

キーワード: protein transduction domain human immunodeficiency virus-1 trans-actuvatir if transcription factor (TAT), peptidyl prolyl cis/trans isomerase Pin1, cell cycle

ジャーナルフリー早期公開

論文ID: 100372

DOI https://doi.org/10.1271/bbb.100372

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 10 p.2067

詳細

抄録

The phenotypes of mice lacking peptidyl prolyl cis/trans isomerase Pin1 (Pin1^−⁄−) indicated that deficient Pin1 might be related to a variety of diseases. We created TAT-Pin1, a fusion protein of human immunodeficiency virus 1 trans-activator of transcription factor with Pin1. Treatment of HeLa cells with TAT-Pin1 increased the ratio of the S phase. Moreover, TAT-Pin1 restored the proliferating function of Pin1^−⁄− mouse embryonic fibroblasts which cannot restart proliferation after G0 arrest. These results indicate that TAT-Pin1 is useful in studying the functions of Pin1 and can be developed as a macromolecular drug for diseases related to Pin1 loss.

責任著者(Corresponding author)

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