Involvement of Both the N-Glycan-relevant and N-Glycan-irrelevant Structural Elements in the Recognition of Human Milk Lactoferrin by the 1CF11 Monoclonal Antibody

Shinichi FUKAYA; Chisato SHINODA; Tomio YABE; Yoshihiro KANAMARU

doi:10.1271/bbb.100405

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Involvement of Both the N-Glycan-relevant and N-Glycan-irrelevant Structural Elements in the Recognition of Human Milk Lactoferrin by the 1CF11 Monoclonal Antibody

Shinichi FUKAYA, Chisato SHINODA, Tomio YABE, Yoshihiro KANAMARU

著者情報

キーワード: human milk lactoferrin, 1CF11 monoclonal antibody, immunoreactivity, N-glycan

ジャーナルフリー早期公開

論文ID: 100405

DOI https://doi.org/10.1271/bbb.100405

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 10 p.2127

詳細

抄録

Monoclonal antibody 1CF11 has been suggested to specifically recognize a certain carbohydrate epitope shared by glycoproteins in human external secretions. We examined the effect of cleaving the polypeptide backbone and removing N-linked oligosaccharides on the reactivity with 1CF11 of human milk lactoferrin (hLf) to elucidate the structural features of the 1CF11 epitope. We reveal by treating hLF with trypsin and/or N-glycosidase that both the N-glycan-relevant and N-glycan-irrelevant structural elements were involved in the recognition of hLf by 1CF11.

責任著者(Corresponding author)

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