Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO; Makiko SAKKA; Tetsuya KIMURA; Khanok RATANAKHANOKCHAI; Kazuo SAKKA

doi:10.1271/bbb.100555

This article has now been updated. Please use the final version.

Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

Maya SUDO, Makiko SAKKA, Tetsuya KIMURA, Khanok RATANAKHANOKCHAI, Kazuo SAKKA

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Keywords: Paenibacillus curdlanolyticus, xylanase, glycoside hydrolase

JOURNAL FREE ACCESS Advance online publication

Article ID: 100555

DOI https://doi.org/10.1271/bbb.100555

The final version of this article is now available: Vol. 74 (2010) , No. 11 p.2358

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Abstract

The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.

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