Binding Specificity of the Recombinant Cytoplasmic Domain of Cordyceps militaris β-1,3-Glucan Synthase Catalytic Subunit

Minoru UJITA; Ryosuke INOUE; Yusuke MAKINO; Yosuke KATSUNO; Hiroki OKUMURA

doi:10.1271/bbb.100660

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Binding Specificity of the Recombinant Cytoplasmic Domain of Cordyceps militaris β-1,3-Glucan Synthase Catalytic Subunit

Minoru UJITA, Ryosuke INOUE, Yusuke MAKINO, Yosuke KATSUNO, Hiroki OKUMURA

著者情報

キーワード: Cordyceps militaris, β-1,3-glucan synthase, catalytic subunit, cytoplasmic domain, binding specificity

ジャーナルフリー早期公開

論文ID: 100660

DOI https://doi.org/10.1271/bbb.100660

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 1 p.171

詳細

抄録

The cytoplasmic domain of the medicinal mushroom Cordyceps militaris β-1,3-glucan synthase catalytic subunit Fks1 was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and was assayed for binding specificity. The recombinant cytoplasmic domain bound specifically to UDP-agarose and lichenan (β-glucan), but not to ADP-agarose, GDP-agarose, or other carbohydrates.

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