A Tolerance Gene for Prenylated Flavonoid Encodes a 26S Proteasome Regulatory Subunit in Sophora flavescens

Nobukazu SHITAN; Yoshihisa KAMIMOTO; Shota MINAMI; Mizuki KUBO; Kozue ITO; Masataka MORIYASU; Kazufumi YAZAKI

doi:10.1271/bbb.100665

This article has now been updated. Please use the final version.

A Tolerance Gene for Prenylated Flavonoid Encodes a 26S Proteasome Regulatory Subunit in Sophora flavescens

Nobukazu SHITAN, Yoshihisa KAMIMOTO, Shota MINAMI, Mizuki KUBO, Kozue ITO, Masataka MORIYASU, Kazufumi YAZAKI

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Keywords: prenylated flavonoids, Sophora flavescens, functional screening, proteasome subunit, 26S proteasome

JOURNAL FREE ACCESS Advance online publication

Article ID: 100665

DOI https://doi.org/10.1271/bbb.100665

The final version of this article is now available: Vol. 75 (2011) , No. 5 p.982

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Abstract

Yeast functional screening with a Sophora flavescens cDNA library was performed to identify the genes involved in the tolerant mechanism to the self-producing prenylated flavonoid sophoraflavanone G (SFG). One cDNA, which conferred SFG tolerance, encoded a regulatory particle triple-A ATPase 2 (SfRPT2), a member of the 26S proteasome subunit. The yeast transformant of SfRPT2 showed reduced SFG accumulation in the cells.

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