Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of <small>D</small>-Amino Acid Oxidase with Vitroscilla Hemoglobin

Young-Man SEO; Yong-Ho KHANG; Hyungdon YUN

doi:10.1271/bbb.110122

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin

Young-Man SEO, Yong-Ho KHANG, Hyungdon YUN

著者情報

キーワード: D-amino acid oxidase, flavohemoprotein, 3-fluoroalanine, unnatural amino acid

ジャーナルフリー早期公開

論文ID: 110122

DOI https://doi.org/10.1271/bbb.110122

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 4 p.820

詳細

抄録

In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The k_cat value VHb-DAAO (47.1 s⁻¹) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s⁻¹). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）