An Assay for Detecting Neutralization of Rotavirus Infection by Quantitative Determination of VP6 Protein Fluorescence Intensity

Abstract

Previous rotavirus infection studies used the focus reduction assay extensively to evaluate cellular responses to viral infection, but this technique has a number of limitations. In this study, we developed a simplified, accurate rotavirus infection assay to evaluate the effects of inhibitory substances on rotavirus infection in vitro by measurement of the fluorescence intensities of stained cells.