1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa

Masakazu TAKAHASHI; Yuta SUGIYAMA; Kyuichi KAWABATA; Yu TAKAHASHI; Kazuhiro IRIE; Akira MURAKAMI; Yoshito KUBO; Kyoichi KOBAYASHI; Hajime OHIGASHI

doi:10.1271/bbb.110414

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1,2-Di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol as a Superoxide Generation Inhibitor from Perilla frutescens var. crispa

Masakazu TAKAHASHI, Yuta SUGIYAMA, Kyuichi KAWABATA, Yu TAKAHASHI, Kazuhiro IRIE, Akira MURAKAMI, Yoshito KUBO, Kyoichi KOBAYASHI, Hajime OHIGASHI

著者情報

キーワード: Perilla frutescens, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG), superoxide, xanthine oxidase, HL-60 cells

ジャーナルフリー早期公開

論文ID: 110414

DOI https://doi.org/10.1271/bbb.110414

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 11 p.2240

詳細

抄録

Using a superoxide (O₂⁻) generation assay system with differentiated HL-60 cells, 1,2-di-O-α-linolenoyl-3-O-β-galactosyl-sn-glycerol (DLGG) was identified as an O₂⁻ generation inhibitor from Perilla frutescens var. crispa (a local variety, kida-chirimen shiso). DLGG suppressed the O₂⁻ level in a dose-dependent manner with an IC₅₀ value of 21 μM, comparable to those of rosmarinic acid (RoA, IC₅₀=29 μM) and caffeic acid (CA, IC₅₀=30 μM). While RoA and CA also dose-dependently inhibited O₂⁻ generation in a xanthine-xanthine oxidase system, DLGG showed no affect in the same system. Thus DLGG appeared to decrease the O₂⁻ level in the HL-60 assay system by mechanisms different from those of RoA and CA, which appeared to act as O₂⁻ scavengers.

責任著者(Corresponding author)

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