Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

Hidekazu KATAYAMA; Kayo NOZAWA; Osamu NUREKI; Yoshiaki NAKAHARA; Hironobu HOJO

doi:10.1271/bbb.110653

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Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo

Hidekazu KATAYAMA, Kayo NOZAWA, Osamu NUREKI, Yoshiaki NAKAHARA, Hironobu HOJO

著者情報

キーワード: Desulfitobacterium hafnience, pyrrolysine, pyrrolysyl-tRNA synthetase

ジャーナルフリー早期公開

論文ID: 110653

DOI https://doi.org/10.1271/bbb.110653

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 1 p.205

詳細

抄録

Pyrrolysine-tRNA^Pyl complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA^Pyl in vitro. In addition, the PylRS/tRNA^Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA^Pyl pair can be used in protein engineering technology.

責任著者(Corresponding author)

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