Differences in the Substrate Specificities and Active-Site Structures of Two α-<small>L</small>-Fucosidases (Glycoside Hydrolase Family 29) from Bacteroides thetaiotaomicron

Haruko SAKURAMA; Erika TSUTSUMI; Hisashi ASHIDA; Takane KATAYAMA; Kenji YAMAMOTO; Hidehiko KUMAGAI

doi:10.1271/bbb.111004

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Differences in the Substrate Specificities and Active-Site Structures of Two α-L-Fucosidases (Glycoside Hydrolase Family 29) from Bacteroides thetaiotaomicron

Haruko SAKURAMA, Erika TSUTSUMI, Hisashi ASHIDA, Takane KATAYAMA, Kenji YAMAMOTO, Hidehiko KUMAGAI

著者情報

キーワード: 1,3-1,4-α-L-fucosidase (EC 3.2.1.111), α-L-fucosidase (EC 3.2.1.51), glycoside hydrolase family 29

ジャーナルフリー早期公開

論文ID: 111004

DOI https://doi.org/10.1271/bbb.111004

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 5 p.1022

詳細

抄録

Recent studies suggest that α-L-fucosidases of glycoside hydrolase family 29 can be divided into two subfamilies based on substrate specificity and phylogenetic clustering. To explore the validity of this classification, we enzymatically characterized two structure-solved α-L-fucosidases representing the respective subfamilies. Differences in substrate specificities are discussed in relation to differences in active-site structures between the two enzymes.

責任著者(Corresponding author)

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