Vba5p, a Novel Plasma Membrane Protein Involved in Amino Acid Uptake and Drug Sensitivity in Saccharomyces cerevisiae

Masamitsu SHIMAZU; Teruhiro ITAYA; Pongsanat PONGCHAROEN; Takayuki SEKITO; Miyuki KAWANO-KAWADA; Yoshimi KAKINUMA

doi:10.1271/bbb.120455

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Vba5p, a Novel Plasma Membrane Protein Involved in Amino Acid Uptake and Drug Sensitivity in Saccharomyces cerevisiae

Masamitsu SHIMAZU, Teruhiro ITAYA, Pongsanat PONGCHAROEN, Takayuki SEKITO, Miyuki KAWANO-KAWADA, Yoshimi KAKINUMA

著者情報

キーワード: Vba5p, plasma membrane, amino acid, transport, 4-nitroquinoline N-oxide

ジャーナルフリー早期公開

論文ID: 120455

DOI https://doi.org/10.1271/bbb.120455

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 10 p.1993

詳細

抄録

Vba5p is closest to Vba3p in the vacuolar transporter for basic amino acids (VBA) family of Saccharomyces cerevisiae. We found that green fluorescence protein (GFP)-tagged Vba5p localized exclusively to the plasma membrane. The uptake of lysine and arginine by whole cells was little affected by deletion of the VBA5 gene, but was stimulated by overexpression of the VBA5 gene. The inhibitory effect of 4-nitroquinoline N-oxide on cell growth was accelerated by expression of the VBA5 gene, and was lessened by the addition of arginine. These results suggest that Vba5p is a plasma membrane protein involved in amino acid uptake and drug sensitivity.

責任著者(Corresponding author)

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