Bio-Nanocapsules for Signal Enhancement of Alkaline Phosphatase-Linked Immunosorbent Assays

Masumi IIJIMA; Mikako YAMAMOTO; Nobuo YOSHIMOTO; Tomoaki NIIMI; Shun'ichi KURODA

doi:10.1271/bbb.120760

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Bio-Nanocapsules for Signal Enhancement of Alkaline Phosphatase-Linked Immunosorbent Assays

Masumi IIJIMA, Mikako YAMAMOTO, Nobuo YOSHIMOTO, Tomoaki NIIMI, Shun'ichi KURODA

著者情報

キーワード: bio-nanocapsules, alkaline phosphatase, horseradish peroxidase, oriented immobilization, cluster formation

ジャーナルフリー早期公開

論文ID: 120760

DOI https://doi.org/10.1271/bbb.120760

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 4 p.843

詳細

抄録

The bio-nanocapsules displaying about 240 molecules of immunoglobulin G Fc-binding Z domains (ZZ-BNCs) enhanced the signals of enzyme-linked immunosorbent assay by tethering the Fc regions of secondary antibodies (Abs), which were eliminated using high-molecular mass enzymes (e.g., alkaline phosphatase). By way of optimizing the distance between enzymes and Abs, ZZ-BNCs improved sensitivity independently of enzymes.

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