Strain-Specific Identification of Bifidobacterium bifidum OLB6378 by PCR

Takayuki TOSHIMITSU; Marie NAKAMURA; Shuji IKEGAMI; Masaki TERAHARA; Hiroyuki ITOU

doi:10.1271/bbb.120803

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Strain-Specific Identification of Bifidobacterium bifidum OLB6378 by PCR

Takayuki TOSHIMITSU, Marie NAKAMURA, Shuji IKEGAMI, Masaki TERAHARA, Hiroyuki ITOU

著者情報

キーワード: Bifidobacterium bifidum OLB6378, strain-specific polymerase chain reaction (PCR) primer, random amplified polymorphic DNA (RAPD)-PCR, clinical fecal samples

ジャーナルフリー早期公開

論文ID: 120803

DOI https://doi.org/10.1271/bbb.120803

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 3 p.572

詳細

抄録

The aim of the present study was to develop a strain-specific polymerase chain reaction (PCR) primer set for the detection of Bifidobacterium bifidum OLB6378 (OLB6378) that can serve as suitable probiotics for infants. The random amplified polymorphic DNA (RAPD)-PCR technique was used to obtain OLB6378-specific PCR products. One OLB6378-specific RAPD-PCR product was obtained after testing 97 RAPD primers, and was sequenced. Thirteen PCR primer sets were designed from the sequence. One PCR primer set was found to amplify one PCR product when genomic DNA of OLB6378 was used as template. The primer set did not amplify any PCR product when the other genomic DNA was used as template. The primer set was tested with 47 strains of B. bifidum and 20 strains of the other Bifidobacterium species. As a result, we developed an OLB6378-specific primer set, one that should be useful not only for the detection of OLB6378 but also for the quantification of OLB6378.

責任著者(Corresponding author)

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