Establishment of a New Detection System for the Dimerization of IRE1α by BiFC Assay

Satoko SHINJO; Etsu TASHIRO; Masaya IMOTO

doi:10.1271/bbb.130107

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Establishment of a New Detection System for the Dimerization of IRE1α by BiFC Assay

Satoko SHINJO, Etsu TASHIRO, Masaya IMOTO

著者情報

キーワード: inositol requiring kinase 1 α (IRE1α), imaging, bimolecular fluorescent complementation (BiFC) assay, unfolded protein response (UPR), small molecule

ジャーナルフリー早期公開

論文ID: 130107

DOI https://doi.org/10.1271/bbb.130107

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 6 p.1333

詳細

抄録

We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）