Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
Eri YUASA
Faculty of Sciences, Kumamoto University
Daisuke SEKI
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
Takeshi KITANO
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
Hisato SAITOH
Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University Faculty of Sciences, Kumamoto University Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University
In this study, we cloned the catalytic domain of the Oryzias latipes sentrin/SUMO-specific protease 1 (OlSENP1-CD) gene and produced the recombinant OlSENP1-CD protein in Escherichia coli. Experimental procedures designed to reveal the ability of the recombinant protein to show deSUMOylating activity in vitro should be helpful in future studies of other SENPs and the SUMO pathway.
