Improvement of Reticuline Productivity from Dopamine by Using Engineered Escherichia coli

Ju-Sung KIM; Akira NAKAGAWA; Yuya YAMAZAKI; Eitaro MATSUMURA; Takashi KOYANAGI; Hiromichi MINAMI; Takane KATAYAMA; Fumihiko SATO; Hidehiko KUMAGAI

doi:10.1271/bbb.130552

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Improvement of Reticuline Productivity from Dopamine by Using Engineered Escherichia coli

Ju-Sung KIM, Akira NAKAGAWA, Yuya YAMAZAKI, Eitaro MATSUMURA, Takashi KOYANAGI, Hiromichi MINAMI, Takane KATAYAMA, Fumihiko SATO, Hidehiko KUMAGAI

著者情報

キーワード: benzylisoquinoline alkaloid, reticuline, (S)-norcoclaurine synthase, monoamine oxidase, microbial production

ジャーナルフリー早期公開

論文ID: 130552

DOI https://doi.org/10.1271/bbb.130552

この記事には本公開記事があります。

The final version of this article is now available: Vol. 77 (2013) , No. 10 p.2166-2168

詳細

抄録

Benzylisoquinoline alkaloids (BIAs) are pharmaceutically important compounds. We have previously devised a reticuline (BIA) production method from dopamine by using Escherichia coli; however, its productivity was relatively low (33 µM, 11 mg/L). We report here, by fine-tuning the method, higher reticuline productivity of 165 µM (54 mg/L), increasing the conversion efficiency by 8-fold. These results are important for developing an efficient route to fermentative reticuline production.

責任著者(Corresponding author)

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