Enzymatic Synthesis of <small>L</small>-Pipecolic Acid by Δ1-Piperideine-2-carboxylate Reductase from Pseudomonas putida

Hisashi MURAMATSU; Hisaaki MIHARA; Mari YASUDA; Makoto UEDA; Tatsuo KURIHARA; Nobuyoshi ESAKI

doi:10.1271/bbb.60125

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Enzymatic Synthesis of L-Pipecolic Acid by Δ¹-Piperideine-2-carboxylate Reductase from Pseudomonas putida

Hisashi MURAMATSU, Hisaaki MIHARA, Mari YASUDA, Makoto UEDA, Tatsuo KURIHARA, Nobuyoshi ESAKI

著者情報

キーワード: Δ¹-piperideine-2-carboxylate reductase, Pseudomonas putida, L-pipecolic acid, Δ¹-piperideine-2-carboxylate

ジャーナルフリー早期公開

論文ID: 60125

DOI https://doi.org/10.1271/bbb.60125

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2006) , No. 9 p.2296

詳細

抄録

L-Pipecolic acid is a chiral pharmaceutical intermediate. An enzymatic system for the synthesis of L-pipecolic acid from L-lysine by commercial L-lysine α-oxidase from Trichoderma viride and an extract of recombinant Escherichia coli cells coexpressing Δ¹-piperideine-2-carboxylate reductase from Pseudomonas putida and glucose dehydrogenase from Bacillus subtilis is described. A laboratory-scale process provided 27 g/l of L-pipecolic acid in 99.7% e.e.

責任著者(Corresponding author)

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