Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

Tomoki TABUCHI; Tomoyuki OKADA; Tetsushi AZUMA; Takashi NANMORI; Takeshi YASUDA

doi:10.1271/bbb.60309

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Posttranscriptional Regulation by the Upstream Open Reading Frame of the Phosphoethanolamine N-Methyltransferase Gene

Tomoki TABUCHI, Tomoyuki OKADA, Tetsushi AZUMA, Takashi NANMORI, Takeshi YASUDA

著者情報

キーワード: choline, phosphoethanolamine N-methyltransferase, posttranscriptional regulation, upstream open reading frame

ジャーナルフリー早期公開

論文ID: 60309

DOI https://doi.org/10.1271/bbb.60309

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2006) , No. 9 p.2330

詳細

抄録

Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5′ untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5′ UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the β-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream β-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.

責任著者(Corresponding author)

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