Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium

Rie ARAKI; Shuichi KARITA; Akiyoshi TANAKA; Tetsuya KIMURA; Kazuo SAKKA

doi:10.1271/bbb.60348

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Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium

Rie ARAKI, Shuichi KARITA, Akiyoshi TANAKA, Tetsuya KIMURA, Kazuo SAKKA

著者情報

キーワード: carbohydrate-binding module, xylanase, thermostability, differential scanning calorimetry (DSC)

ジャーナルフリー早期公開

論文ID: 60348

DOI https://doi.org/10.1271/bbb.60348

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2006) , No. 12 p.3039

詳細

抄録

A family 22 carbohydrate-binding module (CBM22) from Clostridium stercorarium Xylanase10B raised the optimum temperature of the xylanase, but in the remaining activity of heating test, apparently the catalytic module alone showed higher remaining activity. Differential scanning calorimetry showed that CBM22 conferred resistance to thermal unfolding of the enzyme and prevented the enzyme from refolding after thermal unfolding.

責任著者(Corresponding author)

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