Flavonoid Glycosides Extracted from Hop (Hummulus lupulus L.) as Inhibitors of Chemical Mediator Release from Human Basophilic KU812 Cells

Shuichi SEGAWA; Kazuhisa YASUI; Yoshihiro TAKATA; Toshio KURIHARA; Hirotaka KANEDA; Junji WATARI

doi:10.1271/bbb.60384

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Flavonoid Glycosides Extracted from Hop (Hummulus lupulus L.) as Inhibitors of Chemical Mediator Release from Human Basophilic KU812 Cells

Shuichi SEGAWA, Kazuhisa YASUI, Yoshihiro TAKATA, Toshio KURIHARA, Hirotaka KANEDA, Junji WATARI

著者情報

キーワード: hops, histamine release, quercetin, basophile, protein kinase C

ジャーナルフリー早期公開

論文ID: 60384

DOI https://doi.org/10.1271/bbb.60384

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2006) , No. 12 p.2990

詳細

抄録

The antiallergic properties of hop water extract (HWE) were studied by evaluating histamine release from human basophilic KU812 cells induced by calcium ionophore A23187. HWE significantly inhibited histamine release, but boiling water extract and chloroform–methanol extract did not show any inhibitory effect on it. A 50% methanol-eluted fraction separated from HWE by XAD-4 column chromatography (MFH) had a strong inhibitory effect as compared with HWE. Quercetin glycosides and kaempherol glycosides were identified in MFH, of which quercetin glycosides contributed to the inhibition of histamine release. Most quercetin in HWE existed in glycoside form and its quercetin content, obtained by acid hydrolysis, was about 200 μg/g. HWE and MFH significantly inhibited protein kinase C, which plays a pivotal role in the degranulation of chemical mediators. These results indicate that HWE can inhibit type-I allergic reactions.

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