Preparation of N-Acylated Proteins Modified with Fatty Acids Having a Specific Chain Length Using an Insect Cell-Free Protein Synthesis System

Takashi SUZUKI; Masaaki ITO; Toru EZURE; Masamitsu SHIKATA; Eiji ANDO; Toshihiko UTSUMI; Susumu TSUNASAWA; Osamu NISHIMURA

doi:10.1271/bbb.60470

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Preparation of N-Acylated Proteins Modified with Fatty Acids Having a Specific Chain Length Using an Insect Cell-Free Protein Synthesis System

Takashi SUZUKI, Masaaki ITO, Toru EZURE, Masamitsu SHIKATA, Eiji ANDO, Toshihiko UTSUMI, Susumu TSUNASAWA, Osamu NISHIMURA

著者情報

キーワード: N-acylation, insect cell-free protein synthesis system, protein engineering, saturated fatty acid

ジャーナルフリー早期公開

論文ID: 60470

DOI https://doi.org/10.1271/bbb.60470

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 1 p.261

詳細

抄録

To establish a strategy to generate N-acylated proteins modified with fatty acids having a specific chain length, tGelsolin-streptag, an epitope-tagged model protein having an N-myristoylation motif, was synthesized using an insect cell-free protein synthesis system in the presence of acyl-CoA with various fatty acid chain lengths. It was found that the fatty acid species attached to the N-termini fully depended on the acyl-CoA species added to the reaction mixture. N-Acylated proteins with fatty acid chain lengths of 8, 10, 12, and 14 were generated successfully.

責任著者(Corresponding author)

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