Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins

Hiroki MATSUBARA; Sachiko NAKAMURA-TSURUTA; Jun HIRABAYASHI; Mitsuru JIMBO; Hisao KAMIYA; Tomohisa OGAWA; Koji MURAMOTO

doi:10.1271/bbb.60543

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Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins

Hiroki MATSUBARA, Sachiko NAKAMURA-TSURUTA, Jun HIRABAYASHI, Mitsuru JIMBO, Hisao KAMIYA, Tomohisa OGAWA, Koji MURAMOTO

著者情報

キーワード: carbohydrate binding specificity, C-type lectin, frontal affinity chromatography, invertebrate lectin, lectin

ジャーナルフリー早期公開

論文ID: 60543

DOI https://doi.org/10.1271/bbb.60543

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 2 p.513

詳細

抄録

The sugar-binding specificities of C-type lectins isolated from marine invertebrates were investigated by frontal affinity chromatography (FAC) using 100 oligosaccharides. The lectins included BRA-2 and BRA-3, multiple lectins from the hemolymph of the acorn barnacle, Megabalanus rosa, and BRL from the acorn barnacle, Balanus rostatus. The diverse sugar-binding specificities of the C-type lectins were determined by FAC analysis. BRA-2 recognized α2-6 sialylation but not α2-3 sialylation on glycans. On the other hand, BRA-3 showed high affinity for oligosaccharides with α-linked non-reducing terminal galactose, but not for sialylated forms, and BRL showed enhanced recognition activity towards Lewis^x and Lewis^a epitopes.

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