Structural Characterization of N-Glycans of Cauxin by MALDI-TOF Mass Spectrometry and Nano LC-ESI-Mass Spectrometry

Yusuke SUZUKI; Masao MIYAZAKI; Emi ITO; Minoru SUZUKI; Tetsuro YAMASHITA; Hideharu TAIRA; Akemi SUZUKI

doi:10.1271/bbb.60599

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Structural Characterization of N-Glycans of Cauxin by MALDI-TOF Mass Spectrometry and Nano LC-ESI-Mass Spectrometry

Yusuke SUZUKI, Masao MIYAZAKI, Emi ITO, Minoru SUZUKI, Tetsuro YAMASHITA, Hideharu TAIRA, Akemi SUZUKI

著者情報

キーワード: cauxin, glycoproteins, N-glycan, mass spectrometry

ジャーナルフリー早期公開
電子付録

論文ID: 60599

DOI https://doi.org/10.1271/bbb.60599

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 3 p.811

詳細

抄録

Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to ⁸³Asn was a bisecting complex type, Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)(GlcNAcβ1-4)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc.

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