Dynamic Behavior of FCHO1 Revealed by Live-Cell Imaging Microscopy: Its Possible Involvement in Clathrin-Coated Vesicle Formation

Shinji SAKAUSHI; Kimiko INOUE; Hitomi ZUSHI; Kaori SENDA-MURATA; Takashi FUKADA; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.60720

This article has now been updated. Please use the final version.

Dynamic Behavior of FCHO1 Revealed by Live-Cell Imaging Microscopy: Its Possible Involvement in Clathrin-Coated Vesicle Formation

Shinji SAKAUSHI, Kimiko INOUE, Hitomi ZUSHI, Kaori SENDA-MURATA, Takashi FUKADA, Shigenori OKA, Kenji SUGIMOTO

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Keywords: FCHO1, KIAA0290, clathrin, periodic fluctuations (PFs), dual-color imaging

JOURNAL FREE ACCESS Advance online publication

Article ID: 60720

DOI https://doi.org/10.1271/bbb.60720

The final version of this article is now available: Vol. 71 (2007) , No. 7 p.1764

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Abstract

The intracellular behavior of human FCHO1 protein was investigated by live-cell imaging microscopy. The fluorescence intensity of green fluorescent protein (GFP)-FCHO1 fluctuated periodically in a perinuclear region approximately every 100 s, reminding us of the periodic fluctuations of clathrin reported in our recent work. The periodicity of FCHO1 was temporally correlated with that of clathrin, suggesting that FCHO1 is involved in clathrin-coated vesicle formation.

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