Dynamic Behavior of FCHO1 Revealed by Live-Cell Imaging Microscopy: Its Possible Involvement in Clathrin-Coated Vesicle Formation

Shinji SAKAUSHI; Kimiko INOUE; Hitomi ZUSHI; Kaori SENDA-MURATA; Takashi FUKADA; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.60720

Biochemistry & Molecular Biology Notes

Dynamic Behavior of FCHO1 Revealed by Live-Cell Imaging Microscopy: Its Possible Involvement in Clathrin-Coated Vesicle Formation

Shinji SAKAUSHI, Kimiko INOUE, Hitomi ZUSHI, Kaori SENDA-MURATA, Takashi FUKADA, Shigenori OKA, Kenji SUGIMOTO

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Keywords: FCHO1, KIAA0290, clathrin, periodic fluctuations (PFs), dual-color imaging

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2007 Volume 71 Issue 7 Pages 1764-1768

DOI https://doi.org/10.1271/bbb.60720

View "Advance Publication" version (July 7, 2007).

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Abstract

The intracellular behavior of human FCHO1 protein was investigated by live-cell imaging microscopy. The fluorescence intensity of green fluorescent protein (GFP)-FCHO1 fluctuated periodically in a perinuclear region approximately every 100 s, reminding us of the periodic fluctuations of clathrin reported in our recent work. The periodicity of FCHO1 was temporally correlated with that of clathrin, suggesting that FCHO1 is involved in clathrin-coated vesicle formation.

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