TreX from Sulfolobus solfataricus ATCC 35092 Displays Isoamylase and 4-α-Glucanotransferase Activities

Hye-Sun PARK; Jong-Tae PARK; Hee-Kweon KANG; Hyunju CHA; Dong-Sung KIM; Jung-Wan KIM; Kwan-Hwa PARK

doi:10.1271/bbb.70016

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TreX from Sulfolobus solfataricus ATCC 35092 Displays Isoamylase and 4-α-Glucanotransferase Activities

Hye-Sun PARK, Jong-Tae PARK, Hee-Kweon KANG, Hyunju CHA, Dong-Sung KIM, Jung-Wan KIM, Kwan-Hwa PARK

著者情報

Hye-Sun PARK
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
Jong-Tae PARK
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
Hee-Kweon KANG
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
Hyunju CHA
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
Dong-Sung KIM
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University
Jung-Wan KIM
Department of Biology, University of Incheon
Kwan-Hwa PARK
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University

キーワード: Sulfolobus solfataricus ATCC 35092, TreX, cyclization, disproportionation

ジャーナルフリー早期公開

論文ID: 70016

DOI https://doi.org/10.1271/bbb.70016

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 5 p.1348

詳細

抄録

A treX in the trehalose biosynthesis gene cluster of Sulfolobus solfataricus ATCC 35092 has been reported to produce TreX, which hydrolyzes the α-1,6-branch portion of amylopectin and glycogen. TreX exhibited 4-α-D-glucan transferase activity, catalyzing the transfer of α-1,4-glucan oligosaccharides from one molecule to another in the case of linear maltooligosaccharides (G3–G7), and it produced cyclic glucans from amylopectin and amylose like 4-α-glucanotransferase. These results suggest that TreX is a novel isoamylase possessing the properties of 4-α-glucanotransferase.

責任著者(Corresponding author)

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