Enzymatic Synthesis of α-Anomer-Selective <small>D</small>-Glucosides Using Maltose Phosphorylase

Kuniki KINO; Yu SHIMIZU; Shoko KURATSU; Kohtaro KIRIMURA

doi:10.1271/bbb.70117

This article has now been updated. Please use the final version.

Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase

Kuniki KINO, Yu SHIMIZU, Shoko KURATSU, Kohtaro KIRIMURA

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Keywords: maltose phosphorylase, α-glucoside, transglycosylation

JOURNAL FREE ACCESS Advance online publication

Article ID: 70117

DOI https://doi.org/10.1271/bbb.70117

The final version of this article is now available: Vol. 71 (2007) , No. 6 p.1598

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Abstract

A maltose phosphorylase (EC 2.4.1.8; MPase) showed novel acceptor specificity and transferred the glucosyl moiety of maltose not only to sugars but also to various acceptors having alcoholic OH groups. Salicyl alcohol acted as acceptor for MPase from Enterococcus hirae, and the product, salicyl-O-α-D-glucopyranoside (α-SalGlc) was identified. The yield based on supplied salicyl alcohol was 86% (mol/mol).

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