Enzymatic Synthesis of α-Anomer-Selective <small>D</small>-Glucosides Using Maltose Phosphorylase

Kuniki KINO; Yu SHIMIZU; Shoko KURATSU; Kohtaro KIRIMURA

doi:10.1271/bbb.70117

Biochemistry & Molecular Biology Notes

Enzymatic Synthesis of α-Anomer-Selective D-Glucosides Using Maltose Phosphorylase

Kuniki KINO, Yu SHIMIZU, Shoko KURATSU, Kohtaro KIRIMURA

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Keywords: maltose phosphorylase, α-glucoside, transglycosylation

JOURNAL FREE ACCESS

2007 Volume 71 Issue 6 Pages 1598-1600

DOI https://doi.org/10.1271/bbb.70117

View "Advance Publication" version (June 7, 2007).

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Abstract

A maltose phosphorylase (EC 2.4.1.8; MPase) showed novel acceptor specificity and transferred the glucosyl moiety of maltose not only to sugars but also to various acceptors having alcoholic OH groups. Salicyl alcohol acted as acceptor for MPase from Enterococcus hirae, and the product, salicyl-O-α-D-glucopyranoside (α-SalGlc) was identified. The yield based on supplied salicyl alcohol was 86% (mol/mol).

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