Assessment of Prion Inactivation by Combined Use of Bacillus-Derived Protease and SDS

Miyako YOSHIOKA; Yuichi MURAYAMA; Takehiro MIWA; Katsuhiro MIURA; Masuhiro TAKATA; Takashi YOKOYAMA; Koji NISHIZAWA; Shirou MOHRI

doi:10.1271/bbb.70257

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Assessment of Prion Inactivation by Combined Use of Bacillus-Derived Protease and SDS

Miyako YOSHIOKA, Yuichi MURAYAMA, Takehiro MIWA, Katsuhiro MIURA, Masuhiro TAKATA, Takashi YOKOYAMA, Koji NISHIZAWA, Shirou MOHRI

著者情報

キーワード: prion inactivation, protease, scrapie, bioassay, protein misfolding cyclic amplification

ジャーナルフリー早期公開

論文ID: 70257

DOI https://doi.org/10.1271/bbb.70257

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 10 p.2565

詳細

抄録

Prions, infectious agents causing transmissible spongiform encephalopathy, retain infectivity even after undergoing routine sterilization processes. We found that MSK103 protease, identified in our previous study, effectively reduces infectivity and the level of misfolded isoform of the prion protein in scrapie-infected brain homogenates in the presence of SDS. The treatment therefore can be applied to the decontamination of thermolabile instruments.

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