Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU; Atsushi TOYODA; Naoki ONIZAWA; Yutaka NAKAMURA; Hajime MINATO

doi:10.1271/bbb.70290

This article has now been updated. Please use the final version.

Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU, Atsushi TOYODA, Naoki ONIZAWA, Yutaka NAKAMURA, Hajime MINATO

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Keywords: cellulose-binding protein, cellulase, Eubacterium cellulosolvens 5, glycosyl hydrolase family 9

JOURNAL FREE ACCESS Advance online publication

Article ID: 70290

DOI https://doi.org/10.1271/bbb.70290

The final version of this article is now available: Vol. 71 (2007) , No. 10 p.2577

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Abstract

The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-β-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.

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