Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU; Atsushi TOYODA; Naoki ONIZAWA; Yutaka NAKAMURA; Hajime MINATO

doi:10.1271/bbb.70290

Microbiology & Fermentation Technology Notes

Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU, Atsushi TOYODA, Naoki ONIZAWA, Yutaka NAKAMURA, Hajime MINATO

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Keywords: cellulose-binding protein, cellulase, Eubacterium cellulosolvens 5, glycosyl hydrolase family 9

JOURNAL FREE ACCESS

2007 Volume 71 Issue 10 Pages 2577-2580

DOI https://doi.org/10.1271/bbb.70290

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Abstract

The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-β-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.

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