Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU; Atsushi TOYODA; Naoki ONIZAWA; Yutaka NAKAMURA; Hajime MINATO

doi:10.1271/bbb.70290

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Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU, Atsushi TOYODA, Naoki ONIZAWA, Yutaka NAKAMURA, Hajime MINATO

著者情報

キーワード: cellulose-binding protein, cellulase, Eubacterium cellulosolvens 5, glycosyl hydrolase family 9

ジャーナルフリー早期公開

論文ID: 70290

DOI https://doi.org/10.1271/bbb.70290

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 10 p.2577

詳細

抄録

The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-β-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.

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