Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU; Atsushi TOYODA; Naoki ONIZAWA; Yutaka NAKAMURA; Hajime MINATO

doi:10.1271/bbb.70290

Microbiology & Fermentation Technology Notes

Biochemical Characterization of Cellulose-Binding Proteins (CBPA and CBPB) from the Rumen Cellulolytic Bacterium Eubacterium cellulosolvens 5

Miho YOSHIMATSU, Atsushi TOYODA, Naoki ONIZAWA, Yutaka NAKAMURA, Hajime MINATO

著者情報

キーワード: cellulose-binding protein, cellulase, Eubacterium cellulosolvens 5, glycosyl hydrolase family 9

ジャーナルフリー

2007 年 71 巻 10 号 p. 2577-2580

DOI https://doi.org/10.1271/bbb.70290

View "Advance Publication" version (October 7, 2007).

詳細

抄録

The cellulose-binding proteins, CBPA and CBPB, of rumen cellulolytic bacterium Eubacterium cellulosolvens 5 were biochemically characterized, and their properties were compared. Recombinant CBPA and CBPB were a typical 1,4-β-endoglucanase. Both proteins bound to insoluble polysaccharides such as Avicel cellulose, acid swollen cellulose, lichenan, chitin, and oat spelt xylan. On the other hand, only recombinant CBPB bound to agarose and starch.

責任著者(Corresponding author)

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