High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Saori TAKAHASHI; Keishi HATA; Ken-Ichi KIKUCHI; Takeshi GOTOH

doi:10.1271/bbb.70416

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High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Saori TAKAHASHI, Keishi HATA, Ken-Ichi KIKUCHI, Takeshi GOTOH

著者情報

キーワード: renin, prorenin, expression, baculovirus, Sf-9 cells

ジャーナルフリー早期公開

論文ID: 70416

DOI https://doi.org/10.1271/bbb.70416

この記事には本公開記事があります。

The final version of this article is now available: Vol. 71 (2007) , No. 10 p.2610

詳細

抄録

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

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