Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY; Makiko SAKKA; Tetsuya KIMURA; Kazuo SAKKA

doi:10.1271/bbb.70646

This article has now been updated. Please use the final version.

Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica

Saikat CHAKRABORTY, Makiko SAKKA, Tetsuya KIMURA, Kazuo SAKKA

Author information

Keywords: diaphorase, Clostridium thermocellum, Moorella thermoacetica, rubredoxin, flavin reductase

JOURNAL FREE ACCESS Advance online publication

Article ID: 70646

DOI https://doi.org/10.1271/bbb.70646

The final version of this article is now available: Vol. 72 (2008) , No. 3 p.877

Details

Abstract

Two high-molecular-weight rubredoxin genes (hrb) from Clostridium thermocellum and Moorella thermoacetica were expressed in Escherichia coli and their translated products (Hrb) were characterized. M. thermoacetica Hrb showed strong diaphorase activity. In contrast, C. thermocellum Hrb, containing neither FMN nor FAD in the molecule, showed flavin reductase activity with moderate diaphorase activity. Both enzymes were optimally active at about 50 °C.

Corresponding author

Register with J-STAGE for free!