Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO; Yoshikane ITOH; Yoko NISHIZAWA; Yuichiro KEZUKA; Kazushi SUZUKI; Takamasa NONAKA; Takeshi WATANABE

doi:10.1271/bbb.70693

This article has now been updated. Please use the final version.

Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO, Yoshikane ITOH, Yoko NISHIZAWA, Yuichiro KEZUKA, Kazushi SUZUKI, Takamasa NONAKA, Takeshi WATANABE

Author information

Keywords: family 19 chitinase, antifungal activity, Oryza sativa

JOURNAL FREE ACCESS Advance online publication

Article ID: 70693

DOI https://doi.org/10.1271/bbb.70693

The final version of this article is now available: Vol. 72 (2008) , No. 3 p.893

Details

Abstract

To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.

Corresponding author

Register with J-STAGE for free!