Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO; Yoshikane ITOH; Yoko NISHIZAWA; Yuichiro KEZUKA; Kazushi SUZUKI; Takamasa NONAKA; Takeshi WATANABE

doi:10.1271/bbb.70693

Biochemistry & Molecular Biology Notes

Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli

Ryoji MIZUNO, Yoshikane ITOH, Yoko NISHIZAWA, Yuichiro KEZUKA, Kazushi SUZUKI, Takamasa NONAKA, Takeshi WATANABE

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Keywords: family 19 chitinase, antifungal activity, Oryza sativa

JOURNAL FREE ACCESS

2008 Volume 72 Issue 3 Pages 893-895

DOI https://doi.org/10.1271/bbb.70693

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Abstract

To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.

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