Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases

Atsushi KOTAKA; Hiroshi SAHARA; Yoji HATA; Yasuhisa ABE; Akihiko KONDO; Michiko KATO-MURAI; Kouichi KURODA; Mitsuyoshi UEDA

doi:10.1271/bbb.70825

This article has now been updated. Please use the final version.

Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases

Atsushi KOTAKA, Hiroshi SAHARA, Yoji HATA, Yasuhisa ABE, Akihiko KONDO, Michiko KATO-MURAI, Kouichi KURODA, Mitsuyoshi UEDA

Author information

Keywords: sake yeast, Aspergillus oryzae, glucoamylase, cell-surface engineering, bioethanol

JOURNAL FREE ACCESS Advance online publication

Article ID: 70825

DOI https://doi.org/10.1271/bbb.70825

The final version of this article is now available: Vol. 72 (2008) , No. 5 p.1376

Details

Abstract

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.

Corresponding author

Register with J-STAGE for free!