Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases

Atsushi KOTAKA; Hiroshi SAHARA; Yoji HATA; Yasuhisa ABE; Akihiko KONDO; Michiko KATO-MURAI; Kouichi KURODA; Mitsuyoshi UEDA

doi:10.1271/bbb.70825

Microbiology & Fermentation Technology Notes

Efficient and Direct Fermentation of Starch to Ethanol by Sake Yeast Strains Displaying Fungal Glucoamylases

Atsushi KOTAKA, Hiroshi SAHARA, Yoji HATA, Yasuhisa ABE, Akihiko KONDO, Michiko KATO-MURAI, Kouichi KURODA, Mitsuyoshi UEDA

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Keywords: sake yeast, Aspergillus oryzae, glucoamylase, cell-surface engineering, bioethanol

JOURNAL FREE ACCESS

2008 Volume 72 Issue 5 Pages 1376-1379

DOI https://doi.org/10.1271/bbb.70825

View "Advance Publication" version (May 7, 2008).

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Abstract

Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.

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