Reconstitution of Bacteriorhodopsin into Cyclic Lipid Vesicles

Motonari SHIBAKAMI; Hisayoshi TSUIHIJI; Shin MIYOSHI; Makoto NAKAMURA; Rie GOTO; Shigeki MITAKU; Masashi SONOYAMA

doi:10.1271/bbb.80050

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Reconstitution of Bacteriorhodopsin into Cyclic Lipid Vesicles

Motonari SHIBAKAMI, Hisayoshi TSUIHIJI, Shin MIYOSHI, Makoto NAKAMURA, Rie GOTO, Shigeki MITAKU, Masashi SONOYAMA

著者情報

キーワード: cyclic lipid, bacteriorhodopsin, reconstituted membrane

ジャーナルフリー早期公開

論文ID: 80050

DOI https://doi.org/10.1271/bbb.80050

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 6 p.1623

詳細

抄録

Bacteriorhodopsin (bR), a membrane protein that can generate a light-driven proton pump, was successfully reconstituted into vesicles composed of an artificial cyclic lipid that mimics archaeal membrane lipids. Unlike reconstituted bR in 1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicles, the net topology and structure of bR molecules in cyclic lipid vesicles are identical to those in the native purple membrane of Halobacterium salinarum.

責任著者(Corresponding author)

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