Isolation and Identification of a Thermophilic Strain Producing Trehalose Synthase from Geothermal Water in China

Yueming ZHU; Jun ZHANG; Dongsheng WEI; Yufan WANG; Xiaoyun CHEN; Laijun XING; Mingchun LI

doi:10.1271/bbb.80134

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Isolation and Identification of a Thermophilic Strain Producing Trehalose Synthase from Geothermal Water in China

Yueming ZHU, Jun ZHANG, Dongsheng WEI, Yufan WANG, Xiaoyun CHEN, Laijun XING, Mingchun LI

著者情報

キーワード: Meiothermus ruber, trehalose, trehalose synthase (TreS), 16s rRNA gene

ジャーナルフリー早期公開

論文ID: 80134

DOI https://doi.org/10.1271/bbb.80134

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 8 p.2019

詳細

抄録

A slightly thermophilic strain, CBS-01, producing trehalose synthase (TreS), was isolated from geothermal water in this study. According to the phenotypic characteristics and phylogenetic analysis of the 16s rRNA gene sequence, it was identified as Meiothermus ruber. The trehalose synthase gene of Meiothermus ruber CBS-01 was cloned by polymerase chain reaction and sequenced. The TreS gene consisted of 2,895 nucleotides, which specified a 964-amino-acid protein. This novel TreS catalyzed reversible interconversion of maltose and trehalose.

責任著者(Corresponding author)

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